Hifair™ Advanced One Step RT-qPCR Kit _ 11175ESHifair Advanced One Step RT qPCR Kit is a kit for fluorescence quantification based on SYBR Green I dye. Using gene specific primers, reverse transcription and qPCR reactions are completed in one tube, eliminating the need for repeated cap opening and pipetting operations, greatly improving assay efficiency and reducing the risk of contamination. For RNA samples, the kit employs heat resistant Hifair V Reverse Transcriptase for efficient cDNA

cutting at the site where ribonucleotides are incorporated into DNA

then add 10  μL 5×10E3 copies/μL RCL DNA Standard (Std4) and mix well

Frontiers in Immunology

A: Just dilute it with sterile water

102036(IF:5

EN20250702

not for human use

Recommended working concentration is 1-100 μ G/mL

and probably dendritic cells

24 h after transfection

IL-36 alpha (IL1F6)

PI is a nucleic acid dye